The use of botulinum toxins (BoNTs) in healthcare has become very popular, with over 3.9 million Botox procedures being performed in the United States in 2022. In the field of dentistry, BoNT is emerging as a versatile tool for managing orofacial pain (OFP), functional disorders, and aesthetic concerns. The purpose of this Quality Resource Guide is to provide a science-based overview of BoNT’s mechanism of action, applications, benefits, risks, and contraindications in dental practice.